New Genomic Techniques (NGTs): the single-cell gene editing

Abstract

The gene editing technology allows the modification of cellular DNA in crop plants with a high level of precision, creating an alternative route to the classic crossbreeding and to the modern transgene-based approach.
In particular, the use of plant protoplasts (i.e. plant cells in which the cell wall has been removed enzymatically) is characterized by unique opportunities such as the possibility of doing transient and DNA-free transformations with high efficiency, and the possibility to create non-chimeric plants with desirable genetic modifications in elite genotypes.
However, there are also unique challenges that need to be solved before the use of the single cell becomes widely adopted in plant breeding, especially those linked to the in vitro culture and plant regeneration.
Moreover, being a very recent biotechnological approach, the legal framework is still evolving (especially in Europe), adding thus a bit of uncertainty to the future of the technique.
During the seminar, two case studies will be used to better illustrate the topics discussed.

Umerto Salvagnin Curriculum Vitae

Umberto Salvagnin got his MSc in Biotechnology at the University of Padova in 2011 and his PhD from the Free University of Bolzano in 2017. He has worked in plant genetic modification, focusing on studies of chemical ecology, functional genomics, and plant secondary metabolism (terpenes). Currently, he is working as a researcher at Fondazione Edmund Mach in the field of grapevine gene editing.

Umberto Salvagnin - PhD, FEM – Fondazione Edmund Mach
umberto.salvagina@fmach.it