This research is aimed to develop the methods that will eventually allow the screening evaluation of functional medicinal herb extracts enhance cell mediated immunity and against groups of cell line panels which contain multiple and representative hybridoma cell lines.

Our model which stimulated antigen presenting cells (APC) with secretion of Major Histocompatibility Complex (MHC)-I, II helps us for further study of immune effective medicinal herbs (Salsola Laricifolia.L) to spread a broad spectrum on innate immunity and T cell related mechanisms such as activation of CD4, CD8 and biological important cytokines, mediators. As current research, this study demonstrates that these herbal extracts exert their biological effect either through cytotoxic or immune modulating mechanisms and one of most active compounds responsible for the immune effects of herbal products.

In the sorting of T lymphocyte type populations, there appeared no significant differences in cells which were administrated with immune effective SL samples, and cytotoxic effect and mitogenic activity in total splenocyte cultures and monocytic cell line RAW 264.7 in terms of nitric oxide production.

Our present study demonstrates the enhancement of immune response is not involved to the MHC-restricted exogenous antigen presentation with treatment of extract by in vitro. Taken together, Salsola Laricifolia extract has an immune stimulating effect to enhance the cytotoxic activity to target cells and can gives an inhibition on growth of tumor cells and may increase the apoptosis.

Key words:  Cell mediated immunity, major histocompatibility complex, human tumor cell hybridoma, immune enhancing agents, immune medicinal herbs